Twin-focus photothermal correlation spectroscopy method and device for the characterization of dynamical processes in liquids and biomaterials with the help of absorbing markers
Abstract:
The invention relates to a method and a device for twin-focus photothermal correlation spectroscopy for the characterization of dynamical processes in liquids and biomaterials with the help of absorbing markers. Thereby non-fluorescent absorbing nano objects are heated by an intensity-modulated heating laser which leads to a refractive index gradient lens around the object. This refractive index gradient is detected by a detection laser with a focal volume that, depending on the position of the heated object relative to the focal plane of the detection beam, splits into two-sub-volumes forming a twin-focus comprising two sharply separated parts of a focal volume showing no spatial overlap.
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