Gain values of PMTs of a PET scanner's detectors are balanced based on detected radiation from a radioactive calibration source placed in an FOV of the scanner. A time alignment is performed for scintillator crystals of the detectors based on TOF computations based on gamma photons associated with the radioactive calibration source. Baseline data is acquired using intrinsic background radiation of the scintillator crystals, without any object in the FOV. A first set of data, based on the baseline data, is stored in a memory of the scanner. After the acquisition of the baseline data, test data is acquired using intrinsic background radiation of the scintillator crystals, without any object in the FOV. A calibration status of the scanner or of an environment surrounding the scanner is automatically checked based on a comparison between the stored first set of data and a second set of data.