An imaging system may include image processing circuitry and an image sensor having a pixel, readout circuitry, and control circuitry. The pixel may have a dual conversion gain gate for switching between a high conversion gain mode and a low conversion gain mode. The pixel may capture a first image signal while the dual conversion gain gate is turned of and a second image signal subsequent to capturing the first image signal while the dual conversion gain gate is turned on. The readout circuitry may identify a selected one of the first and second image signals to output to the image processing circuitry based on the first image signal. In this way, the readout circuitry may output a low conversion gain signal when saturating charge is stored on the charge storage region and may output a high conversion gain signal when insufficient charge is stored on the charge storage region.