A mobile communication device and an application interface layout switch method are disclosed in the invention. The mobile communication device includes a connection interface unit, a layout library, a display panel and a touch sensing module. The connection interface unit is configured for connecting the mobile communication device to an external device. The layout library is configured for storing a plurality of application interface layout templates. The application interface layout templates are different from each other. The display panel can be configured for displaying according to a selected application interface layout template out of the application interface layout templates. The input sensing module is configured for generating a user input signal based on the selected application interface layout template.