Invention Grant
- Patent Title: Determining the sensitivity of a cell to a drug
- Patent Title (中): 确定细胞对药物的敏感性
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Application No.: US13058035Application Date: 2009-08-10
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Publication No.: US09382568B2Publication Date: 2016-07-05
- Inventor: Vyvyan Clare Salisbury , Habib Mahmoud Alloush , Margaret Ann Smith , Paul John Innocenzi , Mark William Ruddock , Ashley Diane Martin
- Applicant: Vyvyan Clare Salisbury , Habib Mahmoud Alloush , Margaret Ann Smith , Paul John Innocenzi , Mark William Ruddock , Ashley Diane Martin
- Applicant Address: GB GB Bristol
- Assignee: RANDOX LABORATORIES LTD.,UNIVERSITY OF THE WEST OF ENGLAND
- Current Assignee: RANDOX LABORATORIES LTD.,UNIVERSITY OF THE WEST OF ENGLAND
- Current Assignee Address: GB GB Bristol
- Agency: Saliwanchik Lloyd & Eisenschenk
- Priority: GB0814591.4 20080808
- International Application: PCT/GB2009/001969 WO 20090810
- International Announcement: WO2010/015844 WO 20100211
- Main IPC: C12Q1/06
- IPC: C12Q1/06 ; C12Q1/66 ; C12Q1/70 ; G01N33/53 ; G01N33/573 ; C12Q1/02

Abstract:
The present invention provides an in vitro method for determining the resistance or sensitivity of a cell line or patient sample to a deoxyribonucleoside kinase-dependent drug, wherein the method comprises the steps of: (i) treating a patient sample or cell line, or a portion thereof, with a deoxyribonucleoside kinase-dependent drug; (ii) lysing the cells of the patient sample or cell line from step (i); (iii) optionally, mixing a portion of the cell lysate from step (ii) with a bioluminescent reporter bacteria incorporating a gene coding for deoxyribonucleoside kinase; (iv) mixing a portion of the cell lysate from (ii) with a bioluminescent reporter bacteria incorporating a gene coding for a deoxyribonucleoside kinase and a deoxyribonucleoside kinase transcription promoter; (v) mixing a portion of the cell lysate from step (ii) with a bioluminescent reporter bacteria incorporating a gene coding for a deoxyribonucleoside kinase, a deoxyribonucleoside kinase transcription promoter and a dephosphorylating agent; and (vi) measuring the bioluminescence of each of the mixtures from steps (iii) to (v), wherein the comparative levels of bioluminescence of each of the mixtures provides a measure of the resistance or sensitivity to the drug.
Public/Granted literature
- US20110183317A1 DETERMINING THE SENSITIVITY OF A CELL TO A DRUG Public/Granted day:2011-07-28
Information query
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