An optical fiber transmission line capable of minimizing the total splice loss per one span thereof. One span of an optical fiber transmission line provided between repeaters has two connection fibers and (N+1) number of optical transmission fibers. The optical connection fibers and are single mode optical fibers (SSMF) based on International standard ITU-T G.652 standard. (N+1) number of the optical transmission fibers are connected in series in order between the two optical connection fibers. The total splice loss αsp_total of the optical fiber transmission line as calculated from the given formula is 1.4 dB or less, when the average value of the mode field diameter of the two connection fibers is W′, and the average value of the mode field diameter of (N+1) number of the optical transmission fibers is W, and the axial misalignment width at the splice point of the optical transmission fibers is d.