Invention Grant
- Patent Title: Method for detecting toxin-producing Clostridium difficile
- Patent Title (中): 检测产毒梭菌的方法
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Application No.: US14239409Application Date: 2012-08-31
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Publication No.: US09388474B2Publication Date: 2016-07-12
- Inventor: Hiroyuki Kubota , Hiroshi Makino , Takafumi Sakai , Eiji Ishikawa , Kenji Oishi
- Applicant: Hiroyuki Kubota , Hiroshi Makino , Takafumi Sakai , Eiji Ishikawa , Kenji Oishi
- Applicant Address: JP Tokyo
- Assignee: KABUSHIKI KAISHA YAKULT HONSHA
- Current Assignee: KABUSHIKI KAISHA YAKULT HONSHA
- Current Assignee Address: JP Tokyo
- Agency: Oblon, McClelland, Maier & Neustadt, L.L.P
- Priority: JP2011-190706 20110901
- International Application: PCT/JP2012/072219 WO 20120831
- International Announcement: WO2013/031973 WO 20130307
- Main IPC: C12Q1/68
- IPC: C12Q1/68 ; C12P19/34 ; C07H21/04
Abstract:
Provided are an oligonucleotide which realizes specific and highly sensitive detection of toxigenic C. difficile, and a method for detecting toxigenic C. difficile, the method employing the oligonucleotide.1) A primer pair containing an oligonucleotide having a nucleotide sequence represented by SEQ ID NO: 1, and an oligonucleotide having a nucleotide sequence represented by SEQ ID NO: 2; or a primer pair having complementary sequences to the nucleotide sequences. 2) A primer pair containing an oligonucleotide having a nucleotide sequence represented by SEQ ID NO: 4, and an oligonucleotide having a nucleotide sequence represented by SEQ ID NO: 5; or a primer pair having complementary sequences to the nucleotide sequences.
Public/Granted literature
- US20140212879A1 METHOD FOR DETECTING TOXIN-PRODUCING CLOSTRIDIUM DIFFICILE Public/Granted day:2014-07-31
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