Invention Grant
US09389182B2 Labeling of proteins with the fluorophore 7-amino-4-methylcoumarin (AMC) generated novel proteolytic substrates
有权
蛋白质用荧光团标记7-氨基-4-甲基香豆素（AMC）产生新的蛋白水解底物
- Patent Title: Labeling of proteins with the fluorophore 7-amino-4-methylcoumarin (AMC) generated novel proteolytic substrates
- Patent Title (中): 蛋白质用荧光团标记7-氨基-4-甲基香豆素（AMC）产生新的蛋白水解底物
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Application No.: US13600908Application Date: 2012-08-31
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Publication No.: US09389182B2Publication Date: 2016-07-12
- Inventor: Kelvin J. A. Davies , Andrew M. Pickering
- Applicant: Kelvin J. A. Davies , Andrew M. Pickering
- Applicant Address: US CA Los Angeles
- Assignee: University of Southern California
- Current Assignee: University of Southern California
- Current Assignee Address: US CA Los Angeles
- Agency: Brooks Kushman P.C.
- Main IPC: C12P19/46
- IPC: C12P19/46 ; G01N21/64
Abstract:
A method of measuring the degradation of intact proteins includes a step of providing a protein substrate having one or more free or exposed carboxyl groups and then reductively attaching 7-amino-4-methylcoumarin (AMC) to the protein substrate with a reducing agent. The protein substrate is contacted in a test solution with one or more proteolytic enzymes that degrade the protein substrate. The amount of AMC attached to the protein substrate is then determined by monitoring the fluorescence of free 7-amino-4-methylcoumarin that is formed during degradation of the protein substrate to protein fragments.
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