Invention Grant
US09399798B2 3′-OH unblocked, fast photocleavable terminating nucleotides and methods for nucleic acid sequencing
有权
3'-OH未封闭，快速可光切割的终止核苷酸和用于核酸测序的方法
- Patent Title: 3′-OH unblocked, fast photocleavable terminating nucleotides and methods for nucleic acid sequencing
- Patent Title (中): 3'-OH未封闭，快速可光切割的终止核苷酸和用于核酸测序的方法
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Application No.: US14504093Application Date: 2014-10-01
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Publication No.: US09399798B2Publication Date: 2016-07-26
- Inventor: Brian P. Stupi , Hong Li , Weidong Wu , Megan N. Hersh , David Hertzog , Sidney E. Morris , Michael L. Metzker
- Applicant: LASERGEN, INC.
- Applicant Address: US TX Houston
- Assignee: LASERGEN, INC.
- Current Assignee: LASERGEN, INC.
- Current Assignee Address: US TX Houston
- Agency: Parker Highlander PLLC
- Main IPC: C12Q1/68
- IPC: C12Q1/68 ; C07H19/073 ; C07H19/10 ; C07H19/173 ; C07H19/20 ; C07H19/14 ; C07H21/00
Abstract:
The present invention relates generally to 3′-OH unblocked nucleotides and nucleosides labeled and unlabeled with 5-methoxy-substituted nitrobenzyl-based photocleavable terminating groups for use in methods and systems related to DNA and RNA sequencing and analysis. These compounds may be used as reversible terminators as they exhibit fast nucleotide incorporation kinetics, single-base termination, high nucleotide selectivity, and rapid terminating group cleavage that results in a naturally occurring nucleotide.
Public/Granted literature
- US20150176074A1 3'-OH UNBLOCKED, FAST PHOTOCLEAVABLE TERMINATING NUCLEOTIDES AND METHODS FOR NUCLEIC ACID SEQUENCING Public/Granted day:2015-06-25
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