An apparatus and method for removing a Direct Current (DC) offset at a receiving terminal in a wireless communication system are provided. In the method, a frame is divided into at least two time resource blocks. Resource allocation information is used to discriminate between at least one time resource block of a data-unmapped interval and at least one time resource block of a data-mapped interval. The DC offset is measured during the data-unmapped interval. The DC offset is compensated during the data-unmapped interval on a time resource block basis by using the measured DC offset.