An audio control method of an audio player, the audio player includes an audio playing unit having a first channel and a second channel, and an audio control system executed by a processor of the audio player. The audio control system includes an audio playing module, a detection module and a channel adjustment module. The audio playing module plays an audio file and outputs audio signals of the audio file through the audio playing unit, the audio signals have a first channel signals and a second channel signals. The detection module detects whether the first channel signals and the second channel signals are normally played. The channel adjustment module combines one of the first and second channel signals to another of the first and second channel signals, to form combination signals, and plays the combination signals through the first or the second channel.