A tobacco composition for use in a smoking article or a smokeless tobacco composition is provided, the composition including a component derived from a seed of the Nicotiana species. The component can be a seed of the Nicotiana species or a portion thereof in particulate form or in the form of seed isolate derived from a seed of the Nicotiana species. In certain embodiments, the seed isolate is in the form of a chemically transformed seed isolate, the chemical transformation being selected from hydrogenation, acid/base reaction, hydrolysis, thermal treatment, and enzymatic treatment. The invention also provides smoking articles and smokeless tobacco compositions that include the seed components described herein, and methods for preparing a component derived from a seed of the Nicotiana species for addition to a tobacco composition.