Invention Grant
- Patent Title: Cell expression system
- Patent Title (中): 细胞表达系统
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Application No.: US14773287Application Date: 2014-02-17
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Publication No.: US09404078B2Publication Date: 2016-08-02
- Inventor: Noelle Sunstrom
- Applicant: NeuClone Biologics Pty Ltd
- Applicant Address: AU Eveleigh, NSW
- Assignee: NeuClone Biologics Pty Ltd
- Current Assignee: NeuClone Biologics Pty Ltd
- Current Assignee Address: AU Eveleigh, NSW
- Agency: Bozicevic, Field & Francis LLP
- Agent Michael B. Rubin
- Priority: AU2013900804 20130308; AU2013900808 20130308
- International Application: PCT/AU2014/000129 WO 20140217
- International Announcement: WO2014/134657 WO 20140912
- Main IPC: C12N15/67
- IPC: C12N15/67 ; C12N15/85 ; C07K14/61 ; C12N5/073 ; C07K16/24 ; C12N9/06

Abstract:
An expression system for expressing a protein comprising: a eukaryotic host cell carrying a dihydrofolate reductase (DHFR) deficiency; and an expression vector, the expression vector encoding the human growth hormone gene; a expression vector, the expression vector comprising: a eukaryotic selectable marker including a minimal SV 40 early promoter driving expression of a sequence encoding dihydrofolate reductase for complementing the DHFR deficiency in the host cell; a prokaryotic selectable marker conveying Ampicillin resistance to a prokaryotic host cell; a prokaryotic Origin of Replication; a plurality of multiple cloning sites (MCS); and at least one protein expression module comprising: a Simian Vacuolating Virus 40 (SV40) early promoter, inclusive of its 72 bp enhancer repeats; and a rabbit β-globin intron sequence being separable from a SV40 p A sequence by a first multiple cloning site, for receiving a coding sequence and expressing a desired protein therefrom.
Public/Granted literature
- US20160017281A1 CELL EXPRESSION SYSTEM Public/Granted day:2016-01-21
Information query
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