Invention Grant
- Patent Title: Method for production of eosinophil from pluripotent stem cell
- Patent Title (中): 从多能干细胞生产嗜酸性粒细胞的方法
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Application No.: US13990991Application Date: 2011-12-02
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Publication No.: US09404082B2Publication Date: 2016-08-02
- Inventor: Tatsutoshi Nakahata , Kohichiro Tsuji , Feng Ma , Hirohisa Saito , Kenji Matsumoto
- Applicant: Tatsutoshi Nakahata , Kohichiro Tsuji , Feng Ma , Hirohisa Saito , Kenji Matsumoto
- Applicant Address: JP Kyoto-shi, Kyoto
- Assignee: KYOTO UNIVERSITY
- Current Assignee: KYOTO UNIVERSITY
- Current Assignee Address: JP Kyoto-shi, Kyoto
- Agency: Knobbe, Martens, Olson & Bear, LLP
- International Application: PCT/JP2011/077955 WO 20111202
- International Announcement: WO2012/074106 WO 20120607
- Main IPC: C12N5/00
- IPC: C12N5/00 ; C12N5/08 ; C12Q1/02 ; C12N5/0787 ; C12N5/074 ; G01N33/50
Abstract:
The present invention relates to a method for producing human eosinophils from human pluripotent stem cells. More specifically, the present invention provides a method for producing human eosinophils from human pluripotent stem cells, which method comprises the steps of: (1) co-culturing, in the presence of VEGF, human pluripotent stem cells with cells separated from the AGM region of a mammalian fetus; (2) performing suspension culture using a medium comprising IL-3, IL-6, Flt3 ligand, SCF, TPO and serum; (3) performing suspension culture using a medium comprising IL-3, SCF, GM-CSF and serum; and, optionally, (4) performing suspension culture using a medium comprising IL-3, IL-5 and serum.
Public/Granted literature
- US20150104820A1 METHOD FOR PRODUCTION OF EOSINOPHIL FROM PLURIPOTENT STEM CELL Public/Granted day:2015-04-16
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