Invention Grant
- Patent Title: Integration of genes into the chromosome of Saccharopolyspora spinosa
- Patent Title (中): 将基因整合到多孢子虫的染色体中
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Application No.: US13100220Application Date: 2011-05-03
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Publication No.: US09404107B2Publication Date: 2016-08-02
- Inventor: Lei Han , Nigel Mouncey
- Applicant: Lei Han , Nigel Mouncey
- Applicant Address: US IN Indianapolis
- Assignee: Dow AgroSciences LLC
- Current Assignee: Dow AgroSciences LLC
- Current Assignee Address: US IN Indianapolis
- Agency: Magleby Cataxinos & Greenwood
- Agent Yung H. Lee
- Main IPC: C12P21/06
- IPC: C12P21/06 ; C12N15/75 ; C12N15/10 ; C12P19/62 ; C12P21/00 ; C12N15/52 ; C12N15/90
Abstract:
This invention includes a process for the identification and validation of a neutral polynucleotide integration site within the S. spinosa genome. In addition, the invention includes the use of the neutral site and methods for the integration of a polynucleotide containing a gene expression cassette, which is stably maintained and expressed over subsequent generations. The invention includes neutral integration sites that can be disrupted without negatively impacting spinosyn production, growth or other desired metabolic characteristics.
Public/Granted literature
- US20120282624A1 INTEGRATION OF GENES INTO THE CHROMOSOME OF SACCHAROPOLYSPORA SPINOSA Public/Granted day:2012-11-08
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