An optical projection and image sensing apparatus including a light source, a light valve, a first lens set, a sensing module, and a beam splitter is provided. The light valve is used to convert an illumination light from the light source to an image light beam. The first lens set is used to project the image light to display an image on a screen, and the sensing module is used to sense a sensing light from the image on the screen. The beam splitter is disposed on the optical paths of the image light and the sensing light from the image on the screen. One of the sensing module and the light valve is disposed on the optical path of the sensing light passing through the beam splitter, and the other is disposed on optical path of the sensing light reflected by the beam splitter.