A lighting system with adjustable wavelength output that may include a plurality of light sources, a controller positioned in electrical communication with the plurality of light sources, and an optical sensor system positioned in electrical communication with the controller. The controller may be configured to selectively operate each light source of the plurality of light sources to emit one or more source lights. The optical sensor system may be configured to sense a reflected light that is reflected by an object upon which the source lights are incident. The optical sensor system may be configured to sense a wavelength range of the reflected light defined as a measured wavelength range and may further be configured to transmit a signal to the controller responsive to the measured wavelength range. The controller may be configured to operate the plurality of light sources responsive to the signal received from the optical sensor system.