A scrambling sequence generation method and apparatus of a device supporting Device-to-Device communication are provided. The method includes acquiring at least one of an identifier of the device, an identifier of a counterpart device and a preconfigured value; determining an initial value of the scrambling sequence for the Device-to-Device communication based on the at least one of the identifier of the device, the identifier of the counterpart device, and the preconfigured value; and generating the scrambling sequence using the initial value.