A method of tracking motion of at least one object of a group of moving objects using hyperspectral imaging includes, among other things, obtaining a series of hyperspectral image frames; comparing each frame in the series to a template to determine changes in the image between frames; identifying a group of pixels in each frame associated with the changes; identifying changes as motion of the moving objects; correlating the pixel groups frame to frame to spatially determine at least one parameter of the motion of the objects; and correlating the pixel groups with a spectral reflectance profile associated with the at least one object wherein the track of the at least one object is distinguishable from the tracks of other moving objects.