Methods, systems, and computer readable media for testing an air interface device using per user equipment (UE) channel noise are disclosed. One method includes, generating uplink signals at a network equipment test device to be transmitted from plural simulated UEs to an air interface device under test. The method further includes generating and applying per-UE channel noise to the signals, where applying per-UE channel noise includes applying different channel noise to at least some of the uplink signals. The method further includes transmitting the uplink signals with the per-UE channel noise to the air interface device under test.