A wing locking assembly for an agricultural implement is provided. One agricultural implement includes a first frame section having a mounted bracket and a second frame section pivotally coupled to the first frame section. A cam is mounted to the second frame section. The implement includes an actuating cylinder having a first end pivotally coupled to the second frame section, and a second end disposed within a slot of the bracket. The implement also includes a locking mechanism rotatably coupled to the bracket. The locking mechanism includes a first end configured to engage the second end of the actuating cylinder, and a second end configured to abut the cam. The locking mechanism is configured to drive the second end of the actuating cylinder into a desired position within the slot upon contact between the cam and the second end of the locking mechanism.