A line separation protector is provided that can include an arm, an anchor, a clasp attached to the arm, and a tab connected to and configured to manipulate the clasp. A tubing subsystem is also provided that can include a first tubing threaded through the anchor of the line separation protector described herein. A line connection system is also provided and can include the tubing subsystem described herein, and a second tubing, wherein the clasp of the line separation protector is configured to engage the second tubing. A method of protecting a line connection system is also described herein and includes engaging a second tubing with the clasp of the protector to form a protected line connection system.