Invention Grant
- Patent Title: Chemoenzymatic glycoengineering of antibodies and Fc fragments thereof
- Patent Title (中): 抗体的化学糖工程化及其Fc片段
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Application No.: US14376248Application Date: 2013-02-11
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Publication No.: US09434786B2Publication Date: 2016-09-06
- Inventor: Lai-Xi Wang , Wei Huang
- Applicant: UNIVERSITY OF MARYLAND, BALTIMORE
- Applicant Address: US MD Baltimore
- Assignee: UNIVERSITY OF MARYLAND, BALTIMORE
- Current Assignee: UNIVERSITY OF MARYLAND, BALTIMORE
- Current Assignee Address: US MD Baltimore
- Agency: Moore & Van Allen, PLLC
- Agent Marianne Fuierer
- International Application: PCT/US2013/025553 WO 20130211
- International Announcement: WO2013/120066 WO 20130815
- Main IPC: C07K16/28
- IPC: C07K16/28 ; C07K16/18 ; C12N9/24 ; A61K47/48 ; C07K16/00
Abstract:
The present invention provides for recombinant Endo-S mutants that exhibit reduced hydrolysis activity and increased transglycosylation activity for the synthesis of glycoproteins wherein a desired sialylated oxazoline or synthetic oligosaccharide oxazoline is added to a core fucosylated or nonfucosylated GlcNAc-protein acceptor. Such recombinant Endo-S mutants are useful for efficient glycosylation remodeling of IgG1-Fc domain to provide different antibody glycoforms carrying structurally well-defined Fc N-glycans.
Public/Granted literature
- US20150087814A1 CHEMOENZYMATIC GLYCOENGINEERING OF ANTIBODIES AND FC FRAGMENTS THEREOF Public/Granted day:2015-03-26
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