Invention Grant
- Patent Title: Heat stable keratinase and use thereof
- Patent Title (中): 热稳定角蛋白酶及其用途
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Application No.: US14903194Application Date: 2014-07-17
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Publication No.: US09434934B2Publication Date: 2016-09-06
- Inventor: Meng-Chiao Ho , Shih-Hsiung Wu , Wan-Ling Wu
- Applicant: Academia Sinica
- Applicant Address: TW Taipei
- Assignee: ACADEMIA SINICA
- Current Assignee: ACADEMIA SINICA
- Current Assignee Address: TW Taipei
- Agency: Intellectual Property Connections, Inc.
- Agent Hsiu-Ming Saunders
- International Application: PCT/US2014/047052 WO 20140717
- International Announcement: WO2015/017137 WO 20150205
- Main IPC: C12N9/58
- IPC: C12N9/58 ; C12N9/52 ; C07K14/39 ; C12N15/52 ; C12P21/06
Abstract:
A fusion gene encoding M. taiwanensis WR-220 keratinase is disclosed. The fusion comprises: (a) a first DNA sequence encoding a protein secretion signal peptide, located at the N-terminus of the fusion gene; (b) a second DNA sequence encoding an inhibitory domain of M. taiwanensis WR-220 keratinase, linked in translation frame with the first DNA sequence; and (c) a third DNA sequence encoding a catalytic domain of M. taiwanensis WR-220 keratinase, linked in translation frame with the second DNA sequence, wherein the fusion gene is a non-naturally occurring chimeric DNA. Also disclosed are a method for preparation of the catalytic domain of M. taiwanensis WR-220 keratinase, and use of the M. taiwanensis WR-220 keratinase.
Public/Granted literature
- US20160145593A1 HEAT STABLE KERATINASE AND USE THEREOF Public/Granted day:2016-05-26
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