A DNA chip for diagnosis of genitourinary infection, and in particular, a DNA chip for diagnosis of genitourinary infection on which oligonucleotide probes for detecting fourteen genitourinary infection pathogens are immobilized is provided. The DNA chip for diagnosis of genitourinary infections may accurately and rapidly analyze infections of fourteen genitourinary infections including multiple infections in various samples with high sensitivity, high specificity, and high reproducibility. Accordingly, the DNA chip may be used for diagnosis and treatment of genitourinary infections in primary health care institutions.