Invention Grant
- Patent Title: Recombinant fusion antigen gene, recombinant fusion antigen protein and subunit vaccine composition having the same against infection of porcine reproductive and respiratory syndrome virus
- Patent Title (中): 重组融合抗原基因,与猪繁殖与呼吸综合征病毒感染相同的重组融合抗原蛋白和亚单位疫苗组合物
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Application No.: US14667697Application Date: 2015-03-25
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Publication No.: US09441015B2Publication Date: 2016-09-13
- Inventor: Mei-Li Wu , Hso-Chi Chaung , Guan-Ming Ke
- Applicant: NATIONAL PINGTUNG UNIVERSITY OF SCIENCE AND TECHNOLOGY
- Applicant Address: TW Pingtung
- Assignee: NATIONAL PINGTUNG UNIVERSITY OF SCIENCE AND TECHNOLOGY
- Current Assignee: NATIONAL PINGTUNG UNIVERSITY OF SCIENCE AND TECHNOLOGY
- Current Assignee Address: TW Pingtung
- Agency: CKC & Partners Co., Ltd.
- Priority: TW103140120A 20141119
- Main IPC: C07K14/005
- IPC: C07K14/005 ; C12N7/00 ; A61K39/39 ; A61K39/12 ; A61K39/00

Abstract:
The present invention is directed to a recombinant fusion antigen gene, a recombinant fusion antigen protein and a subunit vaccine composition having the same against infection of porcine reproductive and respiratory syndrome virus (PRRSV). A recombinant fusion antigen gene, which encodes glycoprotein GP5 with truncated N′-terminal decoy epitope, a linker sequence and membrane protein M, followed by codon optimization, is expressed by a baculovirus expression system in vitro, thereby enhancing a yield of the recombinant fusion antigen protein. The recombinant fusion antigen protein can be applied in a subunit vaccine composition, for providing vaccinated animals with better protection ability without the risks of virulent spread and virulent recovery.
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