Invention Grant
US09441227B2 Methods and compositions for the specific inhibition of gene expression by double-stranded RNA
有权
通过双链RNA特异性抑制基因表达的方法和组合物
- Patent Title: Methods and compositions for the specific inhibition of gene expression by double-stranded RNA
- Patent Title (中): 通过双链RNA特异性抑制基因表达的方法和组合物
-
Application No.: US14537578Application Date: 2014-11-10
-
Publication No.: US09441227B2Publication Date: 2016-09-13
- Inventor: John J. Rossi , Mark A. Behlke , Dongho Kim
- Applicant: CITY OF HOPE , INTEGRATED DNA TECHNOLOGIES, INC.
- Applicant Address: US CA Duarte US IA Coralville
- Assignee: CITY OF HOPE,INTEGRATED DNA TECHNOLOGIES, INC.
- Current Assignee: CITY OF HOPE,INTEGRATED DNA TECHNOLOGIES, INC.
- Current Assignee Address: US CA Duarte US IA Coralville
- Agency: Rothwell, Figg, Ernst & Manbeck P.C.
- Main IPC: C07H21/04
- IPC: C07H21/04 ; C12N15/113 ; C12N15/11
Abstract:
The invention is directed to compositions and methods for selectively reducing the expression of a gene product from a desired target gene in a cell, as well as for treating diseases caused by the expression of the gene. More particularly, the invention is directed to compositions that contain double stranded RNA (“dsRNA”), and methods for preparing them, that are capable of reducing the expression of target genes in eukaryotic cells. The dsRNA has a first oligonucleotide sequence that is between 25 and about 30 nucleotides in length and a second oligonucleotide sequence that anneals to the first sequence under biological conditions. In addition, a region of one of the sequences of the dsRNA having a sequence length of at least 19 nucleotides is sufficiently complementary to a nucleotide sequence of the RNA produced from the target gene to trigger the destruction of the target RNA by the RNAi machinery.
Public/Granted literature
- US20150110860A1 METHODS AND COMPOSITIONS FOR THE SPECIFIC INHIBITION OF GENE EXPRESSION BY DOUBLE-STRANDED RNA Public/Granted day:2015-04-23
Information query
