Invention Grant
- Patent Title: Detecting single nucleotide polymorphism using overlapping hydrolysis probes
- Patent Title (中): 使用重叠水解探针检测单核苷酸多态性
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Application No.: US14555343Application Date: 2014-11-26
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Publication No.: US09441268B2Publication Date: 2016-09-13
- Inventor: Rochak Mehta
- Applicant: Roche Molecular Systems, Inc.
- Applicant Address: US CA Pleasanton
- Assignee: Roche Molecular Systems, Inc.
- Current Assignee: Roche Molecular Systems, Inc.
- Current Assignee Address: US CA Pleasanton
- Agent David J. Chang; M. Reza Savari
- Main IPC: C12Q1/68
- IPC: C12Q1/68

Abstract:
Methods for the rapid detection of the presence or absence of a SNP in a target nucleic acid in a sample are described. The methods can include performing an amplifying step, a hybridizing step utilizing a double stranded probe with two overlapping SNP specific hydrolysis probe sequences where one of the probe sequences can include a hairpin structure toward the 3′ end, and a detecting step. Furthermore, the double stranded SNP specific hydrolysis probes along with kits are provided that are designed for the detection of a SNP in a target nucleic acid.
Public/Granted literature
- US20160145673A1 DETECTING SINGLE NUCLEOTIDE POLYMORPHISM USING OVERLAPPING HYDROLYSIS PROBES Public/Granted day:2016-05-26
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