Methods for calibrating multiple electromagnetic radiation detectors within a detection system and methods of using such calibrated systems which include generating a calibration electromagnetic radiation beam at a first temperature. At least a portion of the calibration electromagnetic radiation beam is detected with a first electromagnetic radiation detector. An average intensity value of a plurality of pixels from the first electromagnetic radiation detector is obtained. One or more pixels of the first electromagnetic radiation detector are adjusted to decrease the difference between the intensity of an individual pixel and the average intensity value of the first electromagnetic radiation detector. Detecting at least a portion of the calibration electromagnetic radiation beam with a second electromagnetic radiation detector. One or more pixels of the second electromagnetic radiation detector are adjusted to decrease the difference between the intensity of an individual pixel and the average intensity value of the first electromagnetic radiation detector.