Invention Grant
- Patent Title: Phage display using contranslational translocation of fusion polypeptides
- Patent Title (中): 噬菌体展示使用融合多态的翻译易位
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Application No.: US14468597Application Date: 2014-08-26
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Publication No.: US09447142B2Publication Date: 2016-09-20
- Inventor: Daniel Steiner , Patrik Forrer , Michael T. Stumpp , Andreas Pluckthun
- Applicant: University of Zurich
- Applicant Address: CH Zurich
- Assignee: University of Zurich
- Current Assignee: University of Zurich
- Current Assignee Address: CH Zurich
- Agency: Finnegan, Henderson, Farabow, Garrett & Dunner
- Priority: EP05106236 20050708
- Main IPC: C40B40/02
- IPC: C40B40/02 ; C07K1/04 ; C12N15/10 ; C12N15/63 ; C12N15/62

Abstract:
The present invention relates to a filamentous phage display method wherein the polypeptides of interest displayed on the phage particle are cotranslationally translocated across the cytoplasmic membrane of Gram-negative bacteria based on the signal recognition particle pathway. This method is particularly suitable for polypeptides, which are known to be difficult to display on phages, and for proteins of cDNA libraries and other combinatorial libraries, in particular when derived from very fast folding, stable protein scaffolds. The invention further relates to phage or phagemid vectors useful in the method comprising a gene construct coding for a fusion polypeptide comprising the polypeptide to be displayed on the phage particle and an N-terminal signal sequence promoting cotranslational translocation.
Public/Granted literature
- US20150057186A1 PHAGE DISPLAY USING COTRANSLATIONAL TRANSLOCATION OF FUSION POLYPEPTIDES Public/Granted day:2015-02-26
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