Invention Grant
US09458191B2 Nanolipoprotein particles and related methods and systems for protein capture, solubilization, and/or purification
有权
纳升脂蛋白颗粒以及用于蛋白质捕获，增溶和/或纯化的相关方法和系统
- Patent Title: Nanolipoprotein particles and related methods and systems for protein capture, solubilization, and/or purification
- Patent Title (中): 纳升脂蛋白颗粒以及用于蛋白质捕获，增溶和/或纯化的相关方法和系统
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Application No.: US14536513Application Date: 2014-11-07
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Publication No.: US09458191B2Publication Date: 2016-10-04
- Inventor: Brett A. Chromy , Paul Henderson , Paul D. Hoeprich, Jr.
- Applicant: LAWRENCE LIVERMORE NATIONAL SECURITY, LLC
- Applicant Address: US CA Livermore
- Assignee: Lawrence Livermore National Security, LLC
- Current Assignee: Lawrence Livermore National Security, LLC
- Current Assignee Address: US CA Livermore
- Agency: Steinfl & Bruno LLP
- Main IPC: C07K1/14
- IPC: C07K1/14 ; C12N11/02 ; C12P3/00
Abstract:
Provided herein are methods and systems for assembling, solubilizing and/or purifying a membrane associated protein in a nanolipoprotein particle, which comprise a temperature transition cycle performed in presence of a detergent, wherein during the temperature transition cycle the nanolipoprotein components are brought to a temperature above and below the gel to liquid crystalling transition temperature of the membrane forming lipid of the nanolipoprotein particle.
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