Invention Grant
- Patent Title: Method of producing recombinant ADAMTS13 in cell culture
- Patent Title (中): 在细胞培养中生产重组ADAMTS13的方法
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Application No.: US13179399Application Date: 2011-07-08
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Publication No.: US09458222B2Publication Date: 2016-10-04
- Inventor: Leopold Grillberger , Manfred Reiter , Daniel Fleischanderl , Gregor Bramberger
- Applicant: Leopold Grillberger , Manfred Reiter , Daniel Fleischanderl , Gregor Bramberger
- Applicant Address: US IL Bannockburn CH Glattpark (Opfikon)
- Assignee: Baxalta Incorporated,Baxalta GmbH
- Current Assignee: Baxalta Incorporated,Baxalta GmbH
- Current Assignee Address: US IL Bannockburn CH Glattpark (Opfikon)
- Agency: Morgan, Lewis & Bockius LLP
- Main IPC: C07K14/755
- IPC: C07K14/755 ; C12N9/64 ; A61K38/00
Abstract:
Among other aspects, the present invention relates to cell culture conditions for producing high molecular weight vWF, in particular, highly multimericWF with a high specific activity and ADAMTS13 with a high specific activity. The cell culture conditions of the present invention can include, for example, a cell culture medium with an increased copper concentration and/or cell culture supernatant with a low ammonium (NH4+) concentration. The present invention also provides methods for cultivating cells in the cell culture conditions to express high molecular weight vWF and rA13 having high specific activities.
Public/Granted literature
- US20120034674A1 METHOD OF PRODUCING RECOMBINANT ADAMTS13 IN CELL CULTURE Public/Granted day:2012-02-09
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