Invention Grant
US09458479B2 Method for late introduction of the (8R) hydroxyl group carbapenem β-lactam antibiotic synthesis
有权
晚期引入（8R）羟基碳青霉烯β-内酰胺抗生素合成方法
- Patent Title: Method for late introduction of the (8R) hydroxyl group carbapenem β-lactam antibiotic synthesis
- Patent Title (中): 晚期引入（8R）羟基碳青霉烯β-内酰胺抗生素合成方法
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Application No.: US14860103Application Date: 2015-09-21
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Publication No.: US09458479B2Publication Date: 2016-10-04
- Inventor: Craig Arthur Townsend , Micah Jeffrey Bodner , Ryan Martin Phelan , Michael Francis Freeman
- Applicant: THE JOHNS HOPKINS UNIVERSITY
- Applicant Address: US MD Baltimore
- Assignee: THE JOHNS HOPKINS UNIVERSITY
- Current Assignee: THE JOHNS HOPKINS UNIVERSITY
- Current Assignee Address: US MD Baltimore
- Agency: Ward and Smith, P.A.
- Agent Jeffrey W. Childers
- Main IPC: C07D477/20
- IPC: C07D477/20 ; C12P17/10 ; C12P17/18
Abstract:
The presently disclosed subject matter demonstrates that ThnG and ThnQ enzymes encoded by the thienamycin gene cluster in Streptomyces cattleya oxidize the C-2 and C-6 moieties of carbapenems, respectively. ThnQ stereospecifically hydroxylates PS-5 giving N-acetyl thienamycin. ThnG catalyzes sequential desaturation and sulfoxidation of PS-5, giving PS-7 and its sulfoxide. The ThnG and ThnQ enzymes are relatively substrate selective, but give rise to the oxidative diversity of carbapenems produced by S. cattleya, and orthologues likely function similarly in allied streptomyces.
Public/Granted literature
- US20160083760A1 METHOD FOR LATE INTRODUCTION OF THE (8R) HYDROXYL GROUP CARBAPENEM BETA-LACTAM ANTIBIOTIC SYNTHESIS Public/Granted day:2016-03-24
Information query
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