The present invention relates to a tissue collection system. The system includes an outer housing, a first cylindrical fenestrated member within and immovable relative to the outer housing, a second cylindrical fenestrated member defining an inner tissue collection chamber and being positioned within and rotatable relative to the first cylindrical fenestrated member, a plunger axially movable within the second fenestrated member, an elongate rod being connected to the plunger, and one or more stop units attached to the outer housing. The present invention also relates to methods for separating components of a tissue sample using a system according to the present invention.