Invention Grant
US09481865B2 Serum-free freezing medium used in adipose-derived stem cells and establishment of adipose-derived stem cell library
有权
用于脂肪干细胞的无血清冷冻培养基和建立脂肪来源的干细胞文库
- Patent Title: Serum-free freezing medium used in adipose-derived stem cells and establishment of adipose-derived stem cell library
- Patent Title (中): 用于脂肪干细胞的无血清冷冻培养基和建立脂肪来源的干细胞文库
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Application No.: US14369448Application Date: 2012-08-06
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Publication No.: US09481865B2Publication Date: 2016-11-01
- Inventor: Helen Zhang , Luyi Zhang , Wei Cao
- Applicant: Helen Zhang , Luyi Zhang , Wei Cao
- Applicant Address: CN Shanghai CN Wuxi, Jiangsu
- Assignee: Cellular Biomedicine Group (Shanghai) Ltd,Cellular Biomedicine Group (Wuxi) Ltd
- Current Assignee: Cellular Biomedicine Group (Shanghai) Ltd,Cellular Biomedicine Group (Wuxi) Ltd
- Current Assignee Address: CN Shanghai CN Wuxi, Jiangsu
- Agency: Hamre, Schumann, Mueller & Larson, P.C.
- Priority: CN201110227449 20110809
- International Application: PCT/CN2012/079737 WO 20120806
- International Announcement: WO2013/020492 WO 20130214
- Main IPC: C12N5/071
- IPC: C12N5/071 ; A01N1/02 ; C12N5/00
Abstract:
Disclosed is a serum-free freezing medium used in adipose-derived stem cells and a method for establishing an adipose-derived stem cell library. The serum-free freezing medium comprises a serum-free culture medium, dimethyl sulfoxide and a serum substitution component KSR; the defects of unstable freezing quality of the adipose-derived stem cells and influence of harmful factors in serum on the adipose-derived stem cells are solved, and the adipose-derived stem cells stored have the advantages of high survival percentage, well adherence growth and strong differentiation capacity.
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