A bicycle rim examination device includes a body having a base and two upright side frames extend from the base. Each of the two side frames has a first cylinder, a second cylinder and a third cylinder connected to the top thereof. Each of the second and third cylinders is connected with a roller. A power supply device is located on one end of the body and has transmission shaft which has a roller and is driven by a motor. The transmission shaft is supported by two slides which are movable along two guide frames. A first detector with a first probe and a second detector with a second probe are connected to the body. The bicycle rim examination device examines the tension of the spokes and the roundness of the rim.