Embodiments of the invention provide a parallax barrier and a display device. The parallax barrier comprises: first and second substrates; liquid crystal, filled between the two substrates; a plurality of strip-like electrode units, disposed parallel to each other and at an interval on a side of the first substrate close to the liquid crystal; a first alignment layer, disposed above the strip-like electrode units; a second alignment layer, disposed on a surface of the second substrate opposed to the first substrate, wherein, a aligning direction of the first alignment layer is perpendicular to an extending direction of the strip-like electrode units, and aligning directions of the first and second alignment layer are opposite, wherein, after the strip-like electrode units are energized, the liquid crystal directly above the strip-like electrode units are rotated by an angle ranged from 85° to 95° in a plane parallel to the substrates.