An optical touching assembly, including a display device, a sensing device, and a touching object, is provided. The display device has a touching area. The sensing device is electrically connected to the display device. The touching object is adapted to enter the touching area, such that the sensing device senses the motion of the touching object to control the display device. The touching object has an optical controlling element that generates light along with the motion of the touching object. The sensing device senses a characteristic variation of the light to trigger at least one operating function of the display device.