Invention Grant
- Patent Title: Methods for providing sialylated oligosaccharides
- Patent Title (中): 提供唾液酸化寡糖的方法
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Application No.: US14363856Application Date: 2012-12-06
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Publication No.: US09539270B2Publication Date: 2017-01-10
- Inventor: Erik Sallomons , Maarten Hotse Wilbrink , Peter Sanders , Johannis Paulus Kamerling , Catherina Anna van Vuure , Johannes Adrianus Hage
- Applicant: Friesland Brands B.V. , Rijksuniversiteit Groningen , Darling Ingredients Nederland B.V.
- Applicant Address: NL Groningen
- Assignee: RIJKSUNIVERSITEIT GRONINGEN
- Current Assignee: RIJKSUNIVERSITEIT GRONINGEN
- Current Assignee Address: NL Groningen
- Agency: Hoffmann & Baron, LLP
- Priority: NL2007931 20111207
- International Application: PCT/NL2012/050857 WO 20121206
- International Announcement: WO2013/085384 WO 20130613
- Main IPC: C08B37/00
- IPC: C08B37/00 ; A61K31/7028 ; A61K31/702 ; A61K31/715 ; C12P19/26 ; C12P19/28 ; C07H3/06 ; C12P19/00 ; C12P19/04 ; C12P19/14

Abstract:
The invention relates to a method for providing analogs of human milk oligosaccharides (HMO), in particular oligosaccharides containing terminal sialic acid. The method comprises the steps of: a) providing a source of non-digestible galactooligosaccharides (GOS) containing at least two terminally bonded β-linked galactose residues; b) providing a sialic acid donor having (α2-3)-sialylated O-glycans; c) contacting said GOS with said sialic acid donor in the presence of an enzyme having trans-sialidase activity in an enzyme reaction mixture; and d) isolating from said enzyme reaction mixture a fraction comprising at least 20 percent by weight of disialylated galactooligosaccharides (di-Sia-GOS) based on the dry matter.
Public/Granted literature
- US20140336140A1 METHODS FOR PROVIDING SIALYLATED OLIGOSACCHARIDES Public/Granted day:2014-11-13
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