The present invention relates to a multi-touch positioning method and a multi-touch screen. The multi-touch positioning method comprising: emitting, by a first infrared ray generator set at a first angle of a display panel, infrared rays at a first wavelength; emitting, by a second infrared ray generator set at a second angle, infrared rays at a second wavelength; receiving the infrared rays of the first wavelength and generating a first infrared ray image by a first infrared ray image sensor set at an opposite angle of the first angle; receiving the infrared rays of the second wavelength and generating a second infrared ray image by a second infrared ray image sensor set at an opposite angle of the second angle; and performing processings for the first infrared ray image and the second infrared ray image to determine at least one touch point.