Invention Grant
US09546358B2 Compositions and methods for reducing background DNA amplification
有权
用于减少背景DNA扩增的组合物和方法
- Patent Title: Compositions and methods for reducing background DNA amplification
- Patent Title (中): 用于减少背景DNA扩增的组合物和方法
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Application No.: US13799463Application Date: 2013-03-13
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Publication No.: US09546358B2Publication Date: 2017-01-17
- Inventor: Nathan Tanner , Thomas C. Evans
- Applicant: New England Biolabs, Inc.
- Applicant Address: US MA Ipswich
- Assignee: New England Biolabs, Inc.
- Current Assignee: New England Biolabs, Inc.
- Current Assignee Address: US MA Ipswich
- Agency: New England Biolabs, Inc.
- Agent Harriet M. Strimpel
- Main IPC: C12N9/12
- IPC: C12N9/12 ; C12Q1/68
Abstract:
Compositions are provided that include a plurality of small molecules selected from the group consisting of an amide, urea or acetone having a molecular weight less than 300 g/mol; and dNTPs and a polymerase in a buffer suitable for use as an amplification buffer. Methods of use of the compositions are also described for reducing non-template DNA amplification.
Public/Granted literature
- US20130323793A1 Compositions and Methods for Reducing Background DNA Amplification Public/Granted day:2013-12-05
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