Invention Grant
- Patent Title: Use of anticoagulants in the production of recombinant proteins in the milk of transgenic animals
- Patent Title (中): 在转基因动物牛奶中使用抗凝剂生产重组蛋白
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Application No.: US13582044Application Date: 2011-03-11
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Publication No.: US09546381B2Publication Date: 2017-01-17
- Inventor: Kathryn Margaret Nelson , Michael William Mosesson , Anthony Pusateri , Erik Jordahl Forsberg
- Applicant: Kathryn Margaret Nelson , Michael William Mosesson , Anthony Pusateri , Erik Jordahl Forsberg
- Applicant Address: NL
- Assignee: PHARMING INTELLECTUAL PROPERTY BV
- Current Assignee: PHARMING INTELLECTUAL PROPERTY BV
- Current Assignee Address: NL
- Agency: Alston & Bird LLP
- Priority: EP10156751 20100317
- International Application: PCT/NL2011/050166 WO 20110311
- International Announcement: WO2011/115479 WO 20110922
- Main IPC: C12N15/85
- IPC: C12N15/85 ; A61K31/37 ; C07K14/75
Abstract:
The invention relates to the production of recombinant human fibrinogen (rhFib) in the milk of transgenic mammalian animals. This production and subsequent purification process is generally hampered by the occurrence of so-called ‘clots’ and ‘flakes’ in the milk which, in severe cases, may prevent the cow from being milked at all, resulting in a halt of lactation. These clots and flakes occur because of the expression of the fibrinogen protein, which is a factor that is normally involved in blood clotting. The invention relates to solving this milk clotting problem by treating the (lactating) animals with anticoagulants such as coumarins. A preferred anticoagulant that is used in the methods of the present invention is warfarin.
Public/Granted literature
- US20130053546A1 Use of Anticoagulants in the Production of Recombinant Proteins in the Milk of Transgenic Animals Public/Granted day:2013-02-28
Information query
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