A surgical template positioning device is provided, and has a template and a dental mold. The template has a first surface formed with at least three spherical recesses, a second surface, and a guiding hole. The dental mold has at least three spherical portions partially embedded in the at least three spherical recesses and an implanted hole having an axis the same as the guiding hole. The at least three spherical recesses have three spherical centers as being three vertices of a triangle, the axis passes through the triangle, and the at least three spherical portions have spherical centers corresponding with the three vertices of the triangle. A method of manufacturing the surgical positioning device is also provided.