An apparatus comprises a shaft and an end effector. The end effector is positioned at the distal end of the shaft and is operable to form a fastener assembly to secure tissue. The end effector includes first and second fastener assembly forming features. The first fastener assembly forming feature is rotatable relative to the shaft. The fastener assembly is formed by first and second fastener assembly members. The first fastener assembly member comprises a buttress strip received by the first fastener assembly forming feature. The second fastener assembly member comprises either a wire with protruding portions or a fastener strip with integral protruding fasteners. The buttress strip receives the protruding portions or protruding fasteners to secure tissue between the buttress strip and the wire or fastener strip. The end effector may transect layers of tissue and fasten the layers of tissue together on each side of the transection.