Invention Grant
- Patent Title: Method for preparing eptifibatide
- Patent Title (中): 埃替非巴肽的制备方法
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Application No.: US14392203Application Date: 2014-06-16
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Publication No.: US09562071B2Publication Date: 2017-02-07
- Inventor: Xu Kang , Jian Liu , Yaping Ma , Jiancheng Yuan
- Applicant: HYBIO PHARMACEUTICAL CO., LTD.
- Applicant Address: CN Shenzhen
- Assignee: HYBIO PHARMACEUTICAL CO., LTD.
- Current Assignee: HYBIO PHARMACEUTICAL CO., LTD.
- Current Assignee Address: CN Shenzhen
- Agency: Parker Highlander PLLC
- Priority: CN201310263341 20130627
- International Application: PCT/CN2014/079935 WO 20140616
- International Announcement: WO2014/206210 WO 20141231
- Main IPC: A61K38/04
- IPC: A61K38/04 ; C07K5/00 ; C07K7/00 ; C07K16/00 ; C07K17/00 ; C07K7/06

Abstract:
Provided is a method for preparing eptifibatide, wherein the method comprises: obtaining an eptifibatide refined peptide solution; and obtaining and freeze-drying an eptifibatide refined peptide concentrate after rotary evaporation of the eptifibatide refined peptide solution, wherein the concentration of the eptifibatide refined peptide concentrate is 15-30 mg/ml and the temperature of rotary evaporation is 251° C.-35° C. The preparation method of the eptifibatide refined peptide solution is as follows: coupling Cys with a resin to obtain a Cys-resin; obtaining a polypeptide having a sequence as represented by SEQ ID NO: 1 by gradually coupling the Cys-resin with Pro, Trp, Asp, Gly, Har and Mpa; and obtaining the eptifibatide refined peptide solution through oxidation, cleavage, purification and transfer to salt.
Public/Granted literature
- US20160222059A1 METHOD FOR PREPARING EPTIFIBATIDE Public/Granted day:2016-08-04
Information query
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