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US09567647B2 Primer and probe for detection of Mycobacterium avium and method for detection of Mycobacterium avium by using the primer or probe 有权
用于检测鸟分枝杆菌的引物和探针以及使用引物或探针检测鸟分枝杆菌的方法

Primer and probe for detection of Mycobacterium avium and method for detection of Mycobacterium avium by using the primer or probe
Abstract:
The object of the present invention is to provide a novel primer for use in the detection of Mycobacterium avium (M. avium), and a method for detection of M. avium conveniently, rapidly and with high precision by using the primer. More specifically, disclosed are an oligonucleotide which comprises a part or an entire of the nucleotide sequence selected from SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:37, SEQ ID NO:38, SEQ ID NO:39, SEQ ID NO:40, SEQ ID NO:41, SEQ ID NO:42, SEQ ID NO:130, SEQ ID NO:131, SEQ ID NO:132, SEQ ID NO:133, SEQ ID NO:134, SEQ ID NO:135 and SEQ ID NO:136, or a part or an entire of the sequence complementary to the nucleotide sequence, and which is capable of hybridizing with the nucleotide sequence for a Mycobacterium avium gene; a primer and a probe for use in the detection of M. avium, which comprises the oligonucleotide; and a method for detection of M. avium using the primer and/or probe.
Abstract(Chinese):

本发明的目的是提供一种用于检测鸟分枝杆菌（鸟分枝杆菌）的新型引物，以及通过使用引物，快速且高精度地检测鸟分枝杆菌的方法。 更具体地，公开了包含选自SEQ ID NO：1，SEQ ID NO：2，SEQ ID NO：3，SEQ ID NO：4，SEQ ID NO：5， SEQ ID NO：37，SEQ ID NO：38，SEQ ID NO：39，SEQ ID NO：40，SEQ ID NO：41，SEQ ID NO：42，SEQ ID NO：130，SEQ ID NO：131， NO：132，SEQ ID NO：133，SEQ ID NO：134，SEQ ID NO：135和SEQ ID NO：136，或与核苷酸序列互补的部分或全部序列，并且能够与 鸟分枝杆菌基因的核苷酸序列; 用于检测鸟分枝杆菌的引物和探针，其包含寡核苷酸; 以及使用引物和/或探针检测鸟分枝杆菌的方法。

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