An optical fiber connection structure is provided, in which a first optical fiber and a second optical fiber, which is optically connected to the first optical fiber via a solid refractive index-matching material which is provided on a tip surface of the first optical fiber, are held and fixed between a base member and a cover member, a tip of the second optical fiber is disposed so as to come into contact with the solid refractive index matching material or separated from the solid refractive index-matching material, and the entire solid refractive index matching material and the tip of the second optical fiber are disposed in a liquid refractive index matching agent which is provided between the base member and the cover member.