The invention discloses a regulation jig including a regulation part and a scale plate which are movably connected. The regulation part can simultaneously regulate positions of conveying rings having the same sequence, such that they are arranged into a line. The scale plate can accurately control the distance between the lines formed by the rings. The regulation jig not only can guarantee the straightness of a row of conveying rings around different conveying rollers and having the same sequence, but also can ensure that the transmission surface formed by the conveying rings for supporting and conveying the display substrates has a good flatness, in turn preventing the display substrates from deviating from its travelling direction during the transmission. As such, the regulation jig provided by the invention can efficiently and promptly regulate the positions of the conveying rings, so as to ensure the normal transmission of the display substrate.