Invention Grant
US09574199B2 Simultaneous site-specific integrations of multiple gene-copies in filamentous fungi
有权
在丝状真菌中同时进行多个基因拷贝的位点特异性整合
- Patent Title: Simultaneous site-specific integrations of multiple gene-copies in filamentous fungi
- Patent Title (中): 在丝状真菌中同时进行多个基因拷贝的位点特异性整合
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Application No.: US14118995Application Date: 2012-05-23
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Publication No.: US09574199B2Publication Date: 2017-02-21
- Inventor: Hiroaki Udagawa
- Applicant: Hiroaki Udagawa
- Applicant Address: DK Bagsvaerd
- Assignee: Novozymes A/S
- Current Assignee: Novozymes A/S
- Current Assignee Address: DK Bagsvaerd
- Agent Robert Starnes
- Priority: EP11167051 20110523
- International Application: PCT/EP2012/059597 WO 20120523
- International Announcement: WO2012/160093 WO 20121129
- Main IPC: C12P21/02
- IPC: C12P21/02 ; C12N15/80
Abstract:
The invention relates to a method for the simultaneous integration of two or more copies of a polynucleotide of interest into the chromosome of a fungal host cell comprising at least two pairs of recognition sequences of a site-specific recombinase, each pair flanking a resident negative selection marker; transformation of the cell with a construct carrying a gene of interest also flanked by the recognition sequences to ensure double-crossover events after transient expression of the recombinase, followed by selection for excision of all negative selection markers from the cell.
Public/Granted literature
- US20140120625A1 Simultaneous Site-Specific Integrations of Multiple Gene-Copies in Filamentous Fungi Public/Granted day:2014-05-01
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