Invention Grant
US09574218B2 Method of co-expressing galactosyltransferase and a glycoprotein in a transgenic plant cell and sialylating the glycoprotein for production of glycoprotein having human-type sugar chain
有权
在转基因植物细胞中共表达半乳糖基转移酶和糖蛋白的方法,并且糖化糖蛋白以产生具有人类糖链的糖蛋白的方法
- Patent Title: Method of co-expressing galactosyltransferase and a glycoprotein in a transgenic plant cell and sialylating the glycoprotein for production of glycoprotein having human-type sugar chain
- Patent Title (中): 在转基因植物细胞中共表达半乳糖基转移酶和糖蛋白的方法,并且糖化糖蛋白以产生具有人类糖链的糖蛋白的方法
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Application No.: US14252215Application Date: 2014-04-14
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Publication No.: US09574218B2Publication Date: 2017-02-21
- Inventor: Kazuhito Fujiyama , Tatsuji Seki , Toshiomi Yoshida
- Applicant: Phyton Holdings, LLC
- Applicant Address: US TX Fort Worth
- Assignee: Phyton Holdings, LLC
- Current Assignee: Phyton Holdings, LLC
- Current Assignee Address: US TX Fort Worth
- Agency: Wolf, Greenfield & Sacks, P.C.
- Priority: JP2001-012519 20010119
- Main IPC: C12N15/82
- IPC: C12N15/82 ; C12P21/00

Abstract:
A method for the secretory production of a glycoprotein having a human-type sugar chain, comprising a step of introducing a gene of an enzyme capable of performing a transfer reaction of a galactose residue to a non-reducing terminal acetylglucosamine residue, and a gene of heterologous glycoprotein, to obtain a transformed plant cell, a step of culturing the plant cell, and a step of recovering the culture medium of the plant cell. The method further includes isolating the glycoprotein from the culture medium and contacting the isolated glycoprotein with a sialic acid transferase to add a sialic acid residue attached to the galactose residue in the N-glycan.
Public/Granted literature
- US20140377799A1 METHOD FOR SECRETORY PRODUCTION OF GLYCOPROTEIN HAVING HUMAN-TYPE SUGAR CHAIN USING PLANT CELL Public/Granted day:2014-12-25
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