Invention Grant
US09574218B2 Method of co-expressing galactosyltransferase and a glycoprotein in a transgenic plant cell and sialylating the glycoprotein for production of glycoprotein having human-type sugar chain 有权
在转基因植物细胞中共表达半乳糖基转移酶和糖蛋白的方法,并且糖化糖蛋白以产生具有人类糖链的糖蛋白的方法

Method of co-expressing galactosyltransferase and a glycoprotein in a transgenic plant cell and sialylating the glycoprotein for production of glycoprotein having human-type sugar chain
Abstract:
A method for the secretory production of a glycoprotein having a human-type sugar chain, comprising a step of introducing a gene of an enzyme capable of performing a transfer reaction of a galactose residue to a non-reducing terminal acetylglucosamine residue, and a gene of heterologous glycoprotein, to obtain a transformed plant cell, a step of culturing the plant cell, and a step of recovering the culture medium of the plant cell. The method further includes isolating the glycoprotein from the culture medium and contacting the isolated glycoprotein with a sialic acid transferase to add a sialic acid residue attached to the galactose residue in the N-glycan.
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